Devices and systems for liquid level detection in hematology instruments, and methods related thereto

ABSTRACT

A hematology instrument with liquid level detection for liquid in a container is provided. The hematology instrument includes a probe disposed next to a container site. The probe is displaceable relative to the container site to enter a container when positioned at the container site. Further, the probe is electrically grounded. The hematology instrument includes a capacitive sensor having at least one capacitive electrode disposed next to the container site. The capacitive sensor also has a detection module to detect capacitive changes at the at least one capacitive electrode when the probe contacts liquid in the container. The hematology instrument also includes a calibration module configured to calibrate the capacitive sensor a plurality of times as the probe displaces relative to the container site to enter the container. Kits and methods related thereto are also provided.

CROSS REFERENCE TO RELATED APPLICATIONS

Under 35 U.S.C. §119(e), this application claims priority to the filingdate of U.S. Provisional Patent Application Ser. No. 61/581,457, filedon Dec. 29, 2011, the disclosure of which application is hereinincorporated by reference in its entirety.

BACKGROUND

The present disclosure relates generally to hematology instruments anddetecting liquid levels within containers. For example, containers suchas sample tubes or test tubes are often used to contain liquid specimenssuch as blood plasma. An automatic analyzer may include a probe fortaking up a liquid specimen, for example, and further may include avertically movable mechanism to lower the probe towards a container. Insome instances, the probe may have a nozzle formed therein or otherwisecoupled to a nozzle. Detecting when the probe contacts the liquid may bedesirable. For example, the probe should lower sufficiently to contactthe liquid, in which case detecting when such contact occurs is useful.Furthermore, in some cases, immersion too deep within the liquid may beundesirable—e.g., may cause an adverse effect on the result of analysisof the liquid specimen. In such case, detection of the surface level ofthe liquid is useful. Still further, detection may be important forother purposes—e.g., identifying a liquid level to identify a volume ofliquid within the container, etc.

SUMMARY

In some aspects of the present disclosure, a hematology instrument withliquid level detection for liquid in a container is provided. Thehematology instrument includes a probe disposed next to a containersite. The probe is displaceable relative to the container site to entera container when positioned at the container site. Further, the probe iselectrically grounded. The hematology instrument includes a capacitivesensor having at least one capacitive electrode disposed next to thecontainer site.

In some aspects of the present disclosure, a method of detecting aliquid level in a container for a hematology instrument is provided. Themethod includes providing a liquid level detection unit having acapacitive sensor with at least one capacitive electrode and a detectionmodule to detect capacitive changes at the at least one capacitiveelectrode; and a probe disposed next to the container site, e.g.,proximate to the container site or adjacent to the container site. Theprobe is electrically grounded and is displaceable relative to thecontainer site to enter a container when positioned at the containersite. The method also includes positioning the at least one capacitiveelectrode next to the container site, configuring a calibration moduleto calibrate the capacitive sensor a plurality of times as the probedisplaces relative to the container site, and configuring the detectionmodule to detect a capacitive change at the at least one capacitiveelectrode when the probe contacts liquid in the container.

In some aspects of the present disclosure, a method of detecting aliquid level in a container for a hematology instrument is provided. Themethod includes positioning at least one capacitive electrode of acapacitive sensor next to a container including liquid, and positioninga probe adjacent to an entry point of the container. The probe iselectrically grounded. The method also includes displacing the proberelative to the container to enter the container and contact the liquid,calibrating the capacitive sensor a plurality of times as the probe isdisplaced relative to the container, and detecting a capacitive changeat the capacitive electrodes when the probe contacts the liquid.

BRIEF DESCRIPTION OF THE DRAWINGS

A detailed description of various embodiments of the present disclosureis provided herein with reference to the accompanying drawings, whichare briefly described below. The drawings are illustrative and are notnecessarily drawn to scale. The drawings illustrate various embodimentsof the present disclosure and may illustrate one or more embodiment(s)or example(s) of the present disclosure in whole or in part. A referencenumeral, letter, and/or symbol that is used in one drawing to refer to aparticular element may be used in another drawing to refer to a likeelement.

FIG. 1 illustrates a block diagram of a hematology instrument withliquid level detection for liquid in a container, according to oneembodiment.

FIG. 2 illustrates a diagram of a hematology instrument with liquidlevel detection for liquid in a container, according to one embodiment.

FIG. 3 illustrates a schematic liquid level detection circuit, accordingto one embodiment.

FIG. 4 illustrates a schematic liquid level detection circuit, accordingto one embodiment.

FIGS. 5A-5C illustrates a hematology instrument with liquid leveldetection at different times during liquid level detection, according toone embodiment.

FIGS. 6A-6D illustrates a hematology instrument with liquid leveldetection at different times during liquid level detection, according toone embodiment.

FIGS. 7A-7B illustrates a hematology instrument with liquid leveldetection at different times during liquid level detection, according toone embodiment.

DETAILED DESCRIPTION

Before the embodiments of the present disclosure are described, it is tobe understood that the present disclosure is not limited to particularembodiments described, as such may, of course, vary. It is also to beunderstood that the terminology used herein is for the purpose ofdescribing particular embodiments only, and is not intended to belimiting, since the scope of the embodiments of the present disclosurewill be limited only by the appended claims.

Where a range of values is provided, it is understood that eachintervening value, to the tenth of the unit of the lower limit unlessthe context clearly dictates otherwise, between the upper and lowerlimits of that range is also specifically disclosed. Each smaller rangebetween any stated value or intervening value in a stated range and anyother stated or intervening value in that stated range is encompassedwithin the present disclosure. The upper and lower limits of thesesmaller ranges may independently be included or excluded in the range,and each range where either, neither or both limits are included in thesmaller ranges is also encompassed within the present disclosure,subject to any specifically excluded limit in the stated range. Wherethe stated range includes one or both of the limits, ranges excludingeither or both of those included limits are also included in the presentdisclosure.

In the description of the present disclosure herein, it will beunderstood that a word appearing in the singular encompasses its pluralcounterpart, and a word appearing in the plural encompasses its singularcounterpart, unless implicitly or explicitly understood or statedotherwise. Further, it will be understood that for any given componentdescribed herein, any of the possible candidates or alternatives listedfor that component, may generally be used individually or in combinationwith one another, unless implicitly or explicitly understood or statedotherwise. Additionally, it will be understood that any list of suchcandidates or alternatives, is merely illustrative, not limiting, unlessimplicitly or explicitly understood or stated otherwise.

Various terms are described below to facilitate an understanding of thepresent disclosure. It will be understood that a correspondingdescription of these various terms applies to corresponding linguisticor grammatical variations or forms of these various terms. It will alsobe understood that the present disclosure is not limited to theterminology used herein, or the descriptions thereof, for thedescription of particular embodiments. The publications discussed hereinare provided solely for their disclosure prior to the filing date of theapplication. Nothing herein is to be construed as an admission that theembodiments of the present disclosure are not entitled to antedate suchpublication by virtue of prior invention. Further, the dates ofpublication provided may be different from the actual publication dateswhich may need to be independently confirmed.

The present disclosure relates generally to hematology instruments anddetecting liquid levels within containers. For example, containers suchas sample tubes or test tubes are often used to contain liquid specimenssuch as blood plasma. An automatic analyzer may include a probe fortaking up a liquid specimen, for example, and further may include avertically movable mechanism to lower the probe towards a container. Insome instances, the probe may have a nozzle formed therein or otherwisecouple to a nozzle. Detecting when the probe contacts the liquid may bedesirable. For example, the probe should lower sufficiently to contactthe liquid, in which case detecting when such contacts occurs is useful.Furthermore, in some cases, immersion too deep within the liquid may beundesirable—e.g., may cause an adverse effect on the result of analysisof the liquid specimen. In such case, detection of the surface level ofthe liquid is useful. Still further, detection may be important forother purposes—e.g., identifying a liquid level to identify a volume ofliquid within the container, etc.

As summarized above, in some aspects of the present disclosure, ahematology instrument with liquid level detection for liquid in acontainer is provided. The hematology instrument includes a probedisposed next to a container site. The probe is displaceable relative tothe container site to enter a container when positioned at the containersite. Further, the probe is electrically grounded. The hematologyinstrument includes a capacitive sensor having at least one capacitiveelectrode disposed next to the container site. The capacitive sensoralso has a detection module to detect capacitive changes at the at leastone capacitive electrode when the probe contacts liquid in thecontainer. The hematology instrument also includes a calibration moduleconfigured to calibrate the capacitive sensor a plurality of times asthe probe displaces relative to the container site to enter thecontainer.

FIG. 1 illustrates a block diagram of a hematology instrument withliquid level detection for liquid in a container, according to oneembodiment. Hematology instrument 100 includes a grounded probe 101 andcapacitive sensor 102 that are disposed next to a container site 103such that the capacitive sensor 102 and grounded probe 101 are next to acontainer 120 when placed at the container site 103. The container 120includes liquid 121 therein. The probe 101 contacts the liquid 121 wheninserted within the container 120, which is detected by the capacitivesensor 102.

The probe 101 may be made from any variety of conductive materials thatenable it to be electrically grounded to a common electric potential ofthe instrument 100. For example, the probe 101 may be made of one ormore metals, metal alloys, or a combination thereof. The probe 101 mayalso include non-conductive materials, such as resins or polymericmaterials (e.g., plastics, rubber, etc.) in combination with conductivematerials to provide a probe that is conductive. In some instances, theprobe 101 may include a nozzle formed or coupled to the end of theprobe. The nozzle may be used, for example, to facilitate with liquid tobe drawn within the probe.

The probe 101 is relatively displaceable with respect to the container120 or container site 103. It should be appreciated that the relativedisplacement of the probe 101 with respect to the container 120 andcontainer site 103 may encompass embodiments where the probe 101 ismoved towards the container 120 as well as embodiments where thecontainer 120 is moved towards the probe 101. For example, in oneembodiment, the probe 101 may be aligned vertically over an uprightcontainer 120, such as a sample tube or test tube, for example, andlowered into an open end of a sample tube. For instance, the probe 101may be coupled to a probe arm 106 that is raised and lowered by an armlifter 107 powered by a motor 108. The motor 108 may be connected, forexample, to a controller or processing device (not shown) that controlsthe movement of the probe 101. In other embodiments, the probe 101 mayremain fixed while the container site 103 and container 120 move towardsthe probe 101 such that the probe 101 enters the container 120 andeventually contacts liquid 121 within the container 120.

As stated above, the hematology instrument 100 includes a capacitivesensor 102 having at least one capacitive electrode 111 disposed next tothe container site 103. The one or more capacitive electrodes 111 aredisposed such that it is next to the container 120 when at the containersite 103. The distances between the capacitive electrode 111 and thecontainer 120 may be, for example, less than 5 inches, such as 3 inchesor less, and including 1 inch or less.

In one embodiment, the plurality of capacitive electrodes 111 isdisposed next to the container site 103 with the capacitive electrodes111 disposed next to each other. The distance between the electrodes mayvary, and example distances between electrodes may range from within 2inches of each other, such as within 1 inch of each other, includingwithin a quarter inch of each other. It should be appreciated that otherdistances may also be implemented in other embodiments. In oneembodiment, the capacitive electrodes 111 are disposed one after anotherin the direction of the displacement of the probe 101—e.g., stackedvertically above one another.

In one embodiment, the plurality of capacitive electrodes 111 includes apair of coupled capacitive electrodes and a central capacitive electrodedisposed between the pair of coupled capacitive electrodes. In someinstances, each of the pair of coupled capacitive electrodes is smallerin physical size than the central capacitive electrode. For example, inone embodiment, the pair of capacitive electrodes are thin strips (e.g.,1 inch wide by 0.1 inch in height) positioned above and below a largercentral capacitive electrode (e.g., 1 inch by 1 inch). It should beappreciated that these measurements are exemplary and that othervariations of dimensions may be implemented in other embodiments.

In one embodiment, the plurality of capacitive electrodes 111 includes afirst plurality of coupled capacitive electrodes and a second pluralityof coupled capacitive electrodes alternately disposed between the firstplurality of coupled capacitive electrodes. For example, a firstcapacitive electrode in the first plurality is positioned at the top,followed by a first capacitive electrode of the second pluralityunderneath it, followed by a second capacitive electrode in the firstplurality positioned underneath the first capacitive electrode of thesecond plurality, followed by a second capacitive electrode in thesecond plurality positioned underneath the second capacitive electrodeof the first plurality, and so on and so forth for all capacitiveelectrodes in the first and second plurality.

In one embodiment, each of the capacitive electrodes of the firstplurality is smaller in size than each of the capacitive electrodes ofthe second plurality, similarly as described above. The number ofcapacitive electrodes in each plurality may vary. In some instances,each plurality has the same number of electrodes, and in otherembodiment, the number of electrodes are different. Example number ofelectrodes within one of the pluralities may range from two to thirty,such as five to fifteen electrodes, including seven to twelveelectrodes. In one embodiment, each plurality includes ten capacitiveelectrodes.

The capacitive sensor 102 also has a detection module 112 to detectcapacitive changes at the at least one capacitive electrode 111 when theprobe 101 contacts liquid 121 in the container 120. In one embodiment,the detection module 112 includes a detection circuit having twoone-shot multivibrators coupled to the capacitive electrodes 111. Theoutputs of the multivibrators may be coupled to electronic circuitryproviding an output signal that may be monitored or measured—e.g., by ameter or measuring device. The instrument 100 may include an outputmodule 113, for example, that indicates when the liquid level isdetected and/or provides data representing the liquid level detection.For example, in some instances, the output module 113 may include avisual or audio device that indicates when the liquid is contacted. Thevisual or audio device may be any variety of devices, such as an LED,speaker, etc. In some instances, the output module 113 may include atransmitting device to transmit a signal representing the liquid leveldetection. In some instances, the output module may include memoryand/or a processing device that stores or otherwise processes the signalindicating a liquid level detection.

The hematology instrument 100 includes a calibration module 114configured to calibrate the capacitive sensor 102 a plurality of timesas the probe 101 displaces relative to the container site 103 to enterthe container 120. Calibration module 114 may include calibrationcircuitry that calibrates the capacitive sensor 102 by setting a DCoffset level of the output signal of the detection module 112. It shouldbe appreciated that the calibration module 114 may also include, orcommunicate with, a processing device that is programmed to calibratethe detection module—e.g., by setting or otherwise adjusting a DC offsetof the output signal. For instance, a computer may be coupled to theinstrument 100 to provide instructions to the calibration module 114 asto when to calibrate the capacitive sensor 102. The computer may receiveinput data, such as data regarding the movement of the probe 101, whichmay be used to determine when the capacitive sensor 102 should becalibrated.

The calibration module 114 is configured to calibrate the capacitivesensor 102 a plurality of times as the probe 101 displaces relative tothe container site 103 to enter the container 120. For example, thecalibration module 114 may be configured to calibrate the capacitivesensor 102 a plurality of times after the probe 101 is displacedrelative to the container site 103. Calibration may also occur beforethe probe 101 is displaced relative to the container site 103 in someinstances. In some instances, the calibration module 114 is configuredto calibrate the capacitive sensor 102 after the probe 101 displaces apredetermined distance relative to the container site 103. In someinstances, the calibration module 114 is configured to calibrate thecapacitive sensor 102 after predetermined incremental distances relativeto the container site 103.

The calibration module 114 may be configured to calibrate thecalibration sensor at times with respect to its position relative to thecontainer 120. For example, the calibration module 114 may be configuredto calibrate the capacitive sensor 102 a plurality of times when theprobe 101 is moved into the container 120. For instance, calibration mayoccur before the probe 101 enters the container 120; when the probe 101enters the container 120; or after the probe 101 enters the container120 disposed at the container site 103. In some instances, thecalibration module 114 is configured to calibrate the capacitive sensor102 after the probe 101 displaces a predetermined distance within thecontainer 120 disposed at the container site 103. In some instances, thecalibration module 114 is configured to calibrate the capacitive sensor102 after predetermined incremental distances within the container 120disposed at the container site 103. It should be appreciated that invarious embodiments, the calibration module 114 may be configured tocalibrate the capacitive sensor 102 in a variety of combinations of anyof the times mentioned above.

In some embodiments, the container 120 includes a cap disposed therein,such as to cap the open end of the container 120 (e.g., sample tube ortest tube). The cap may be made from any variety of materials, such aspolymeric materials (e.g., plastics, rubber, etc.), etc., that may beused to plug the open end of the container 120. The cap should includeaccess for the probe 101 to enter the container 120—e.g., via a holedisposed within the cap that enables a cap piercing needle to fitthrough to form a channel in which the probe 101 may travel to enter thecontainer 120. The cap piercing needle may be made partially orcompletely from conductive material and may be grounded to a commonelectric potential. It should be appreciated that in some embodiments,the cap and cap piercing needle may be a formed as a single element.

It should be appreciated that the container 120 may be made from avariety of materials but should enable the capacitive electrodes todetect capacitive changes within the container 120. For example, thecontainer 120 may be a glass or plastics sample tube or test tube. Itshould be appreciated that the shape of the container 120 may vary forthe purpose of analysis intended.

FIG. 2 illustrates a diagram of an instrument for detecting a level ofliquid within a container, according to one embodiment. Instrument 100is shown including conductive probe 101 that inserts into a container120 having liquid 121 therein. The conductive probe 101 is shownconnected to a liquid tube 109 that enables part or all of the liquid121 to be drawn from the container 120 via the probe 101. Further, theconductive probe 101 is electrically grounded to a common potential 104for instrument 100.

The container 120 (e.g., a glass or plastic sample tube or test tube)having liquid 121 within is shown having cap 133 (e.g., a rubberstopper) disposed in the open end of the container 120. The cap 133includes a hole that enables the probe 101 to enter the container 120.The cap 133 may be made of any variety of materials, such as elastomericand/or polymeric materials (e.g., plastic).

A cap piercing needle 105 made of a conductive material and isconfigured to pierce the cap 133—e.g., via the hole disposed in the cap133. For example, the hole in the cap 133 may be completely or partiallyclosed by the elastomeric material when inserted within the container120 and before the cap piercing needle 105 is inserted through the holeof the cap 133. In the embodiment shown, the cap piercing needle 105 iselectrically grounded to the common potential 104 of instrument 100. Itshould be appreciated that in other embodiments, the cap piercing needle105 may be made from any other variety of conductive and/ornon-conductive materials, such as plastics or glass.

As stated above, the cap 103 has a hole formed therein that enables theconductive probe 101 to be inserted into the container. When insertedinto the hole in the cap 133, the conductive probe 101 is pushed orotherwise displaced through an inner lumen of the cap piercing needle105 to enter the container 120. The conductive probe 101 is insertedinto the container 120 such that the conductive probe 101 eventuallycontacts the liquid 121 within the container 120.

In the embodiment shown, conductive probe 101 is coupled to a probe arm106 that is raised and lowered by an arm lifter 107 that is driven bymotor 108. The motor is controlled, for example, by a computer (notshown) that is communicatively coupled to the motor 108.

Instrument 100 includes a capacitive sensor 102 having capacitiveelectrodes 111. The capacitive sensor 102 is disposed next to acontainer site 103 wherein a holder 110 maintains and stabilizes thecontainer 120 in the upright position. The capacitive sensor 102 ispositioned on one side of the container 120 and holder 110 such thatcapacitive electrodes 111 are disposed near the container.

In the embodiment shown, the capacitive electrodes 111 include a centralcapacitive electrode and a pair of coupled capacitive electrodes. Eachof the pair of coupled capacitive electrodes is smaller than the centralcapacitive electrode. The plurality of capacitive electrodes 111 arestacked vertically with respect to one another such that one of thecoupled capacitive electrodes is above the central capacitive electrodeand the other coupled capacitive electrode is below the centralcapacitive electrode.

In use, a container 120 is positioned at a container site 103 for thehematology instrument 100. The container site 103 may include a holder114 that maintains the container 120 a proper position during operation.The probe 101 is positioned adjacent the entry end of the container 120and relatively displaced towards the container 120 such that the probe101 eventually enters the container 120 and continues until touchingliquid 121 that is within the container 120. The probe 101 may be moved,for example, by the arm lifter 107 and motor 108 as described above. Thecontainer 120 may also include a cap 133 and cap piercing needle 105disposed in the open end of the container 120. In such case, the cappiercing needle 105 is inserted through a hole within the cap, and theprobe 101 enters the container via the lumen formed by the cap piercingneedle 105. As the probe 101 is inserted within the container 120, thecalibration module 114 calibrates the capacitive sensor 102 a pluralityof times. The calibration accounts for any capacitive changes on thecapacitive electrodes 111 due to the displacement of the probe 101. Thecalibration may occur at various times or points of the displacement ofthe probe, as mentioned above and described below. When the probetouches the liquid within the container, the capacitance on thecapacitive electrodes 111 changes significantly and is detected by thedetection module 112 of the capacitive sensor. The detection is outputaccordingly by the output module 113.

FIG. 3 illustrates a partial circuit diagram of an instrument fordetecting a level of liquid within a container, according to oneembodiment. Instrument 200 includes capacitive sensor 102 and probe 101disposed next to a container 120 maintained at a container site 103. Theprobe 101 is disposed above the container 120 and is configured to lowerwithin the container 120. The capacitive sensor 102 includes a singlecapacitive electrode 111 that is positioned next to the side of thecontainer 120. The capacitive electrode is approximately one inchsquared in area. As shown in FIG. 3, the circuit includes a capacitiveplate 111 which is positioned next to a container 120 maintained at acontainer site 103 and having liquid 121 therein. A conductive probe 101is electrically grounded and positioned above the container 120. Theprobe 101 is coupled to a probe arm 106 that lowers the probe 101 intothe container 120.

Capacitive electrode 111 is coupled to detection module 112. In theembodiment shown, capacitive electrode 111 is coupled to one terminal ofa first one-shot multivibrator 16. A second one-shot multivibrator 17 isalso provided with an oscillator 18 connected to provide a signal fortriggering both multivibrators simultaneously at a constant rate. Oneexample type of multivibrator suitable for use in the circuit of FIG. 3is CMOS MC 14528 manufactured by Motorola, Inc.

At the time the multivibrators are triggered, the input waveforms of themultivibrators drop to their zero levels and thereafter the waveformsrise exponentially in a positive direction towards a threshold level.The rate at which the outputs from the multivibrators rise is determinedby the relative sizes of the capacitance of the capacitive electrode 111positioned close to the container 120 and a fixed capacitor 22 connectedto a terminal of the multivibrator 17. The capacitive electrode 111 isconnected to the terminal 14 of the multivibrator 16 and forms with theresistor 24 and the potentiometer 25 connected to a source of voltagesuch as the battery 28, the time constant determining circuit. Similarlythe capacitor 22 and the resistor 27 in combination with thepotentiometer 25 determine the time constant for the multivibrator 17.Thus it can be seen that the output or time constant of themultivibrator 17 is constant since the capacitor 22 is constant,however, because the capacitance of the capacitive electrode 111 canvary as the probe 101 contacts liquid 121, the time constant for themultivibrator 16 can change. Furthermore, the capacitive electrode 111can also vary due to the displacement of the probe 101. Calibrating thecapacitive sensor as the probe is displaced towards the liquid 121 willnullify any capacitive changes due to the displaced probe.

Thus, there can be caused a time difference between the termination ofthe output signal of multivibrator 16 and the output signal of themultivibrator 17 as indicated by the time the input waveforms to themultivibrators respectively reach a threshold voltage.

For detection of changes in the time constants of the multivibratoroutput signals, these output signals are fed to the respective terminalsof a NOR gate 30 which, during the time that the outputs from themultivibrators are both below the logic switching level of the NOR gate,i.e. the time difference between the level change of the multivibratorsignals, will produce an output pulse. The peak of the output pulseextending above the threshold voltage of the diode 34 is thus suppliedfor charging a capacitor 35. The capacitor is connected in parallel witha resistor 36 which is of relatively high resistance to allow the chargeon the capacitor 35 to dissipate slowly.

An operational amplifier 37 is connected in a noninverting configurationand receives the voltage corresponding to the charge on the capacitor 35at the positive terminal to supply an amplified voltage output signal toa meter 38. The circuit of the resistors 39 and 40 are provided toenergize the other terminal of the operational amplifier and determinethe voltage gain of the circuit.

The meter 38 indicates the charge on the capacitor 35 which in turnreflects the difference in the time of reaching the threshold voltagefor the output signals of the multivibrators 16 and 17. Since this timeperiod is based upon the relative charges of the capacitors and sincethe charge on capacitive electrode 111 changes when the probe 101contacts the liquid 121, the meter indicates when contact has occurred.That is, so long as the probe does not contact the liquid 121 and thecapacitive sensor calibrated for displacement of the probe 101, thecapacitance of the capacitive electrode 111 remains substantiallyconstant. However with the probe 101 contacting the liquid 121, thecapacitive value of the capacitive electrode 111 changes therebychanging the time constant of the multivibrator 16 resulting in a higherreading on the meter 38 because of the growing difference between thetermination of the output signals of the multivibrators 16 and 17.

A calibration module 114 includes circuitry to calibrate the capacitivesensor 102. For example, the potentiometer 25 is adjusted so that theoutput of the NOR gate is very slightly positive, that is, so that thereis a very slight indication of the transmission of the output above theconduction level of the diode 34 as shown by a low meter reading on themeter 38. Thereafter contact between the probe 101 and liquid 121 willcause the value of the capacitance on the capacitive electrode 111 tochange resulting in a difference in conduction time between themultivibrators as indicated by the meter 38.

FIG. 4 illustrates a partial circuit diagram of an instrument fordetecting a level of liquid within a container, according to oneembodiment. Instrument 200 includes probe 101 and capacitive sensor 102having a plurality of capacitive electrodes 111 disposed next to acontainer 120 maintained at a container site 103. The probe 101 isdisposed above the container 120 and is configured to lower within thecontainer 120. The plurality of electrodes 111 includes a firstplurality of capacitive electrodes 111 a and second plurality ofcapacitive electrodes 111 b. The first plurality 111 a includes Ncapacitive electrodes (e.g., 10 capacitive electrodes) electricallycoupled to each other. Similarly, the second plurality 111 b includes Ncapacitive electrodes (e.g., 10 capacitive electrodes) electricallycoupled to each other. Each of the first plurality 111 a of coupledcapacitive electrodes is shaped as thin strips approximately 1 inch by0.1 inch. Each of the second plurality 111 b of coupled capacitiveelectrodes is larger than the electrodes in the first plurality 111 a,and is approximately 1 inch by 1 inch.

The plurality of capacitive electrodes is coupled to detection module112. Detection module 112 includes a pair of one-shot multivibrators 16Aand 17A are provided which function in the same manner described in theembodiment in FIG. 3. An oscillator 18A is provided for triggering thesemultivibrators and a potentiometer 25A connected to a voltage source V+at terminal 26A is utilized to power the circuit.

As in the previous embodiment in FIG. 3, operation of this circuit alsodetects the change in capacitance of the capacitive electrodes 111 asthe probe 101 contacts the liquid 121. However in the embodiment shownin FIG. 4, there is a plurality of capacitive electrodes 111, whichimprove the response to the change in capacitance from the probe.Accordingly the capacitive electrodes 111 b are alternately positionedbetween a plurality of coupled capacitive electrodes 111 a. Thecapacitive electrodes 111 a are smaller than the capacitive electrodes111 b in total area. In the circuit the time duration of the input ofthe multivibrator 16A is dependent upon the capacitance of thecapacitive electrodes 111 b, the resistance 47 and the setting of thepotentiometer 25A. Similarly the time duration of the input of themultivibrator 17A is dependent upon the capacitance of the capacitiveelectrodes 111 a, the value of the resistor 46 and the setting of thepotentiometer 25A.

As the probe 101 is moved towards the liquid, the output signal of themultivibrators 16A and 17A will change based on capacitive changesreceived by the adjacent capacitive electrodes. The capacitive sensor102 may be calibrated as the probe is displaced to nullify any effectson capacitance of the capacitive electrodes. When the probe 101 contactsliquid 121 within container 120, the capacitance on the capacitiveelectrodes is changed significantly. By use of the multiple capacitorplates in the two multivibrator circuits, a more distinct or sharpwaveform is obtained for detecting the difference in the capacitance ofthe multivibrator circuits caused by the probe 101 contacting the liquid121 in the container 120.

FIGS. 5A-5C illustrate the hematology instrument shown in FIG. 1 duringdifferent times in operation, according to one embodiment. The container120 contains liquid 121 and is capped with a cap 133 (e.g., rubberstopper). A cap piercing needle 105 is inserted within a hole in the cap133. The cap piercing needle 105 is electrically grounded. The container120 is disposed in holder 110 and maintained in an upright position. Thecapacitive sensor 102 is disposed to one side of the container 120 andholder 114. The capacitance of the capacitive electrodes 111 areinfluenced by the objects next to it—e.g., the container and liquidtherein.

In FIG. 5A, a conductive probe 101 is electrically grounded and loweredtoward the container 120 (e.g., by arm lifter 107 and motor 108). Thecapacitive sensor 102 is calibrated by the calibration module 114 beforeor as the conductive probe 101 is about to enter the cap piercing needle105. In FIG. 5B, the conductive probe 101 is inserted within the cappiercing needle 105. The capacitive sensor 102 is then calibrated againby the calibration module 114 to cancel the conductive probe's influenceon the capacitance on the capacitive electrodes 111.

When the conductive probe 101 reaches the surface of the liquid withinthe sample, as shown in FIG. 5C, a large change in capacitive load onthe capacitive electrodes 111 is detected by the detection module 112 inthe capacitive sensor 102. The large change in capacitance occurs whenthe grounded probe 101 contacts the liquid 121. The detection module 112detects the capacitive change on the electrodes 111 and outputs anindication of the detection via output module 113. For example, theoutput module 113 may transmit data representing the liquid leveldetection to a computer, which may store and process the data related tothe liquid level. The computer may then stop the displacement of theprobe, for example, to prevent the probe from going further into theliquid 121.

It should be appreciated that in other embodiments, the capacitivesensor 102 may be calibrated a different number of times than shown inFIGS. 5A-5C. For example, if the liquid level is low, the capacitivesensor 102 may be calibrated a plurality of times as the conductiveprobe 101 is extending through the cap piercing needle towards thesurface of the liquid. For instance, the conductive probe 101 may beincrementally lowered in small distances, with the capacitive sensor 102being calibrated at each increment. In this way, small changes incapacitive loads from the conductive probe 101 are ignored as theconductive probe 101 is extended through the cap piercing needle 105 andcontainer 120 towards the liquid 121.

FIGS. 6A-6D illustrate the hematology instrument shown in FIG. 1 with alow level of liquid in the sample tube, according to one embodiment.Instrument 100 is similarly configured as the instrument 100 shown inFIGS. 5A-5C, except the liquid level in the container 120 in FIGS. 6A-6Dis low. For example, the liquid level may be less than 3 mL, such as 1mL or less.

In FIG. 6A, the conductive probe 101 is lowered toward the container 120by the arm lifter 107 and motor 108, and the capacitive sensor 102 iscalibrated by the calibration module 114 before or as the conductiveprobe 101 is about to enter the cap piercing needle 105, similarly asshown in FIG. 5A.

In FIG. 6B, the conductive probe 101 is inserted within the cap piercingneedle 105, and the capacitive sensor 102 is calibrated again by thecalibration module 114 to cancel the conductive probe's influence on thecapacitance on the capacitive electrodes 111, similarly as shown in FIG.5B.

In FIG. 6C, the conductive probe 101 is extended an additional distancefrom the last calibration in FIG. 6B and again calibrated by thecalibration module 114. In this way, any additional change incapacitance on the capacitive electrodes 111 due to the movement of theconductive probe 101 will be ignored.

In FIG. 6D, the conductive probe 101 reaches the surface of the liquid121 within the container 120 and a large change in capacitance on thecapacitive electrodes 111 is detected by the detection module 112 of thecapacitive sensor 102. The detection module 112 detects the capacitivechange on the electrodes 111 and outputs an indication of the detectionvia output module 113.

It should be appreciated that the capacitive sensor 102 may becalibrated any number of times by the calibration module 114 as it ismoved toward the surface of the liquid 121. In one embodiment, forexample, the conductive probe 101 may be incrementally lowered apredetermined distance with the capacitive sensor 102 being calibratedat each increment by the calibration module 114 until the conductiveprobe 101 reaches the liquid 121 in the container 120.

FIGS. 7A-7B illustrate the hematology instrument shown in FIG. 1 whereinliquid is contained within a pediatric sample tube. The pediatric sampletube 120 includes a small volume of liquid 121 (e.g., 125 microliters).

In FIG. 7A, instrument 100 is similarly configured as the instrument 100shown in FIGS. 5A-5C, except the liquid 121 in the pediatric sample tube120 is positioned in the pediatric sample tube 120 closer to the cap133. In FIG. 7A, the capacitive sensor 102 is calibrated by thecalibration module 114 before or as the conductive probe is about toenter the cap piercing needle 105, similarly as shown in FIG. 5A.

In FIG. 7B, the conductive probe 101 is inserted within the cap piercingneedle 105, and the conductive probe 101 comes in contact with the fluid121 in the pediatric sample tube 120 and a large change in capacitanceon the capacitive electrodes 111 is detected by the detection module 112of the capacitive sensor 102. The detection module 112 detects thecapacitive change on the electrodes 111 and outputs an indication of thedetection via output module 113.

The following paragraphs provide additional example embodiments formethods related to the detection of liquid levels in a container for ahematology instrument.

In some aspects of the present disclosure, a method of detecting aliquid level in a container for a hematology instrument is provided. Themethod includes providing a liquid level detection unit having acapacitive sensor with at least one capacitive electrode and a detectionmodule to detect capacitive changes at the at least one capacitiveelectrode; and a probe disposed next to the container site. The probe iselectrically grounded and is displaceable relative to the container siteto enter a container when positioned at the container site. The methodalso includes positioning the at least one capacitive electrode next tothe container site, configuring a calibration module to calibrate thecapacitive sensor a plurality of times as the probe displaces relativeto the container site, and configuring the detection module to detect acapacitive change at the at least one capacitive electrode when theprobe contacts liquid in the container.

In one embodiment, the positioning of the at least one capacitiveelectrode includes positioning a plurality of capacitive electrodes oneafter another in the direction of the displacement of the probe.

In one embodiment, the positioning of the at least one capacitiveelectrode includes positioning a central capacitive electrode between apair of coupled capacitive electrodes. Each of the pair of coupledcapacitive electrodes is smaller in physical size than the centralcapacitive electrode.

In one embodiment, the positioning of the at least one capacitiveelectrode includes positioning a first plurality of coupled capacitiveelectrodes to alternate between a second plurality of coupled capacitiveelectrodes. In some instances, each of the coupled capacitive electrodesof the first plurality is smaller in size than each of the coupledcapacitive electrodes of the second plurality. In some instances, thefirst plurality and the second plurality of coupled capacitiveelectrodes include seven to twelve capacitive electrodes each.

In one embodiment, the calibration module is configured to calibrate thecapacitive sensor before the probe is displaced relative to thecontainer site. In one embodiment, the calibration module is configuredto calibrate the capacitive sensor after the probe is displaced relativeto the container site. In some instances, the calibration module isconfigured to calibrate the capacitive sensor after the probe displacesa predetermined distance relative to the container site. In oneembodiment, the calibration module is configured to calibrate thecapacitive sensor after predetermined incremental distances relative tothe container site.

In one embodiment, the method includes positioning a container at thecontainer site. In some instances, the calibration module is configuredto calibrate the capacitive sensor before the probe enters thecontainer. In some instances, the calibration module is configured tocalibrate the capacitive sensor after the probe enters the container. Insome instances, the calibration module is configured to calibrate thecapacitive sensor after the probe displaces a predetermined distancewithin the container. In some instances, the calibration module isconfigured to calibrate the capacitive sensor after predeterminedincremental distances within the container.

In one embodiment, the method includes positioning a cap on thecontainer, positioning a cap piercing needle within the cap, andrelatively displacing the probe through the cap piercing needle to enterthe container.

In some aspects of the present disclosure, a method of detecting aliquid level in a container for a hematology instrument is provided. Themethod includes positioning at least one capacitive electrode of acapacitive sensor 102 next to a container including liquid, andpositioning a probe adjacent to an entry point of the container. Theprobe is electrically grounded. The method also includes displacing theprobe relative to the container to enter the container and contact theliquid, calibrating the capacitive sensor 102 a plurality of times asthe probe is displaced relative to the container, and detecting acapacitive change at the capacitive electrodes when the probe contactsthe liquid.

In one embodiment, the positioning of the at least one capacitiveelectrode includes positioning a plurality of capacitive electrodes oneafter another in the direction of the displacement of the probe.

In one embodiment, the positioning of the at least one capacitiveelectrode includes positioning a central capacitive electrode between apair of coupled capacitive electrodes, wherein each of the pair ofcoupled capacitive electrodes is smaller in physical size than thecentral capacitive electrode.

In one embodiment, the positioning of the at least one capacitiveelectrode includes positioning a first plurality of coupled capacitiveelectrodes to alternate between a second plurality of coupled capacitiveelectrodes. In some instances, each of the coupled capacitive electrodesof the first plurality is smaller in size than each of the coupledcapacitive electrodes of the second plurality. In some instances, thefirst plurality and the second plurality of coupled capacitiveelectrodes include seven to twelve capacitive electrodes each.

In one embodiment, the capacitive sensor is calibrated before the probeis displaced relative to the container site. In one embodiment, thecapacitive sensor is calibrated after the probe is displaced relative tothe container site. In some instances, the capacitive sensor iscalibrated after the probe displaces a predetermined distance relativeto the container site. In some instances, the capacitive sensor iscalibrated after predetermined incremental distances relative to thecontainer site.

In one embodiment, the method includes positioning a container at thecontainer site. In some instances, the capacitive sensor is calibratedbefore the probe enters the container. In some instances, the capacitivesensor is calibrated after the probe enters the container. In someinstances, the capacitive sensor is calibrated after the probe displacesa predetermined distance within the container. In some instances, thecapacitive sensor is calibrated after predetermined incrementaldistances within the container.

In one embodiment, the method includes positioning a cap on thecontainer, positioning a cap piercing needle within the cap, andrelatively displacing the probe through the cap piercing needle to enterthe container.

In some aspects of the present disclosure, kits including one or morehematology instruments with liquid level detection for liquid in acontainer are provided. In one embodiment, the kit includes manuals ormarketing products related to the hematology instrument. In oneembodiment, the kit includes a hematology instrument and one or morecontainers, such as test tubes or sample tubes. In one embodiment, thekits include software related to the hematology instrument. For example,the software may be used to program and otherwise operate the hematologyinstrument. For example, the software may be loaded on a computer tooperate with the hematology instrument.

Other embodiments and modifications within the scope of the presentdisclosure will be apparent to those skilled in the relevant art.Various modifications, processes, as well as numerous structures towhich the embodiments of the present disclosure may be applicable willbe readily apparent to those of skill in the art to which the presentdisclosure is directed upon review of the specification. Various aspectsand features of the present disclosure may have been explained ordescribed in relation to understandings, beliefs, theories, underlyingassumptions, and/or working or prophetic examples, although it will beunderstood that the present disclosure is not bound to any particularunderstanding, belief, theory, underlying assumption, and/or working orprophetic example.

It should be understood that some of the techniques introduced above canbe implemented by programmable circuitry programmed or configured bysoftware and/or firmware, or they can be implemented entirely byspecial-purpose “hardwired” circuitry, or in a combination of suchforms. Such special-purpose circuitry (if any) can be in the form of,for example, one or more application-specific integrated circuits(ASICS), programmable logic devices (PLDs), field-programmable gatearrays (FPGAs), etc.

Software or firmware implementing the techniques introduced herein maybe stored on a machine-readable storage medium and may be executed byone or more general-purpose or special-purpose programmablemicroprocessors. A “machine-readable medium”, as the term is usedherein, includes any mechanism that can store information in a formaccessible by a machine (a machine may be, for example, a computer,network device, cellular phone, personal digital assistant (PDA),manufacturing took, any device with one or more processors, etc.). Forexample, a machine-accessible medium includes recordable/non-recordablemedia (e.g., read-only memory (ROM); random access memory (RAM);magnetic disk storage media; optical storage media; flash memorydevices; etc.), etc.

The preceding examples are put forth so as to provide those of ordinaryskill in the art with a complete disclosure and description of how tomake and use the embodiments of the invention, and are not intended tolimit the scope of what the inventors regard as their invention nor arethey intended to represent that the experiments below are all or theonly experiments performed. Efforts have been made to ensure accuracywith respect to numbers used (e.g., amounts, temperature, etc.) but someexperimental errors and deviations should be accounted for. Unlessindicated otherwise, parts are parts by weight, molecular weight isweight average molecular weight, temperature is in degrees Centigrade,and pressure is at or near atmospheric.

That which is claimed is:
 1. A hematology instrument with liquid leveldetection for liquid in a container, comprising: a probe disposed at aposition relative to a container site, wherein the probe is displaceablerelative to the container site upon entering into a container positionedat the container site, and wherein the probe is electrically grounded; acapacitive sensor comprising: at least one capacitive electrode disposednext to the container site, wherein the at least one capacitiveelectrode comprises: a first plurality of coupled capacitive electrodes;and a second plurality of coupled capacitive electrodes alternatelydisposed between the first plurality of coupled capacitive electrodes inthe direction of the displacement of the probe, wherein each of thefirst plurality of coupled capacitive electrodes is smaller in physicalsize than each of the second plurality of coupled capacitive electrodes;and a detection module to detect capacitive changes at the at least onecapacitive electrode when the probe contacts liquid in the container;and a calibration module that calibrates the capacitive sensor aplurality of times as the probe displaces relative to the container siteto enter the container.
 2. The hematology instrument of claim 1, whereinthe first plurality and the second plurality of coupled capacitiveelectrodes include seven to twelve capacitive electrodes each.
 3. Thehematology instrument of claim 1, wherein the calibration modulecalibrates the capacitive sensor before the probe is displaced relativeto the container site.
 4. The hematology instrument of claim 1, whereinthe calibration module calibrates the capacitive sensor after the probeis displaced relative to the container site.
 5. The hematologyinstrument of claim 1, wherein the calibration module calibrates thecapacitive sensor after the probe displaces a predetermined distancerelative to the container site.
 6. The hematology instrument of claim 1,wherein the calibration module calibrates the capacitive sensor afterdisplacement of the probe by predetermined incremental distancesrelative to the container site.
 7. The hematology instrument of claim 1,wherein the calibration module calibrates the capacitive sensor beforethe probe enters the container.
 8. The hematology instrument of claim 1,wherein the calibration module calibrates the capacitive sensor afterthe probe enters the container.
 9. The hematology instrument of claim 1,wherein the calibration module calibrates the capacitive sensor afterthe probe displaces a predetermined distance within the container. 10.The hematology instrument of claim 1, wherein the calibration modulecalibrates the capacitive sensor after predetermined incrementaldistances within the container.
 11. The hematology instrument of claim1, further comprising: a cap disposed on the container; and acap-piercing needle disposed within the cap, wherein the probe isrelatively displaced through the cap-piercing needle to enter thecontainer.
 12. The hematology instrument of claim 1, wherein calibrationof the capacitive sensor is based upon detected changes in thecapacitance of the at least one capacitive electrode as the probe isdisplaced relative to the container site.
 13. A method of detecting aliquid level in a container for a hematology instrument, comprising:providing a liquid level detection unit including: a capacitive sensorwith at least one capacitive electrode and a detection module to detectcapacitive changes at the at least one capacitive electrode; and a probedisposed at a position relative to a container site, wherein the probeis electrically grounded, and wherein the probe is displaceable relativeto the container site upon entering into a container; positioning the atleast one capacitive electrode next to the container site, wherein theat least one capacitive electrode comprises: a first plurality ofcoupled capacitive electrodes; and a second plurality of coupledcapacitive electrodes alternately disposed between the first pluralityof coupled capacitive electrodes in the direction of the displacement ofthe probe, wherein each of the first plurality of coupled capacitiveelectrodes is smaller in physical size than each of the second pluralityof coupled capacitive electrodes, configuring a calibration module tocalibrate the capacitive sensor a plurality of times as the probedisplaces relative to the container site to enter the container; andconfiguring the detection module to detect a capacitive change at the atleast one capacitive electrode when the probe contacts liquid in thecontainer.
 14. The method of claim 13, wherein the first plurality andthe second plurality of coupled capacitive electrodes include seven totwelve capacitive electrodes each.
 15. The method of claim 13, whereinthe calibration module calibrates the capacitive sensor before the probeis displaced relative to the container site.
 16. The method of claim 13,wherein the calibration module calibrates the capacitive sensor afterthe probe is displaced relative to the container site.
 17. The method ofclaim 13, wherein the calibration module calibrates the capacitivesensor after the probe displaces a predetermined distance relative tothe container site.
 18. The method of claim 13, wherein the calibrationmodule calibrates the capacitive sensor after predetermined incrementaldistances relative to the container site.
 19. The method of claim 13,further comprising positioning a container at the container site,wherein the calibration module calibrates the capacitive sensor beforethe probe enters the container.
 20. The method of claim 13, furthercomprising positioning a container at the container site, wherein thecalibration module calibrates the capacitive sensor after the probeenters the container.
 21. The method of claim 13, further comprisingpositioning a container at the container site, wherein the calibrationmodule calibrates the capacitive sensor after the probe displaces apredetermined distance within the container.
 22. The method of claim 13,further comprising positioning a container at the container site,wherein the calibration module calibrates the capacitive sensor afterpredetermined incremental distances within the container.
 23. The methodof claim 13, further comprising: positioning a cap on the container;positioning a cap-piercing needle within the cap; and relativelydisplacing the probe through the cap-piercing needle to enter thecontainer.
 24. The method of claim 13, wherein calibration of thecapacitive sensor is based upon detected changes in the capacitance ofthe at least one capacitive electrode as the probe is displaced relativeto the container site.